Potential antioxidant activity of Lactobacillus fermentum KF7 from virgin coconut oil products in Padang, West Sumatra, Indonesia

Abstract. Syukur S, Safrizayanti, Zulaiha Supriwardi E, Fahmi A, Nurfadilah KK, Purwati E. 2021. Potential antioxidant activity of Lactobacillus fermentum KF7 from virgin coconut oil products in Padang, West Sumatra, Indonesia. Biodiversitas 23: 1628-1634. The isolation and characterization five commercial Virgin Coconut Oil (VCO) circulating the local market Padang city were conducted. In this study, eighteen isolates lactic acid bacteria (LAB) isolated characterized microscopically, biochemically. VCO-LAB Gram-positive bacilli cocci, catalase-negative, homofermentative (except VD was heterofermentative). There three (VB.3, VE.4) that could produce gamma-aminobutyric (GABA). highest concentration GABA (19.5 mg/mL) produced by isolate VB.3 after 72 hours incubation addition 7% MSG. shows with Monosodium glutamate (MSG), 68.13% for 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ABTS 88.02%. addition, GABA-producing be grown media containing 0.2-1.0 mM hydrogen peroxide, lowest optical density value 0.82 nm. affected secondary metabolites such as peptides enzymes (NADH-oxidase, superoxide dismutase, NADH peroxidase non-heme catalase). production obtained or KF7.